Recombinant Human HSPA1A Protein (His Tag) (PDEH100677)
For research use only.
| Synonyms | HSP70-1B, HSP70-2, HSP70.2, heat shock protein family A (Hsp70) member 1A, heat shock protein family A (Hsp70) member 1B |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Ala2-Asp641 |
| Accession | P0DMV8-1 |
| Calculated Molecular Weight | 69.9 kDa |
| Observed Molecular Weight | 72.2 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
| Purity | > 85% as determined by reducing SDS-PAGE. |
| Endotoxin | < 10 EU/mg of the protein as determined by the LAL method |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
| Background | This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. |
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