Recombinant Human NOL3 Protein (GST Tag) (PKSH032828)
For research use only.
| Synonyms | ARC, Apoptosis Repressor With CARD, Muscle-Enriched Cytoplasmic Protein, Myp, NOL3, NOP, Nop30, Nucleolar Protein 3, Nucleolar Protein of 30 kDa |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Ser208 |
| Accession | O60936 |
| Calculated Molecular Weight | 48.9 kDa |
| Observed Molecular Weight | 30-59 kDa |
| Tag | N-GST |
| Bio-activity | Not validated for activity |
| Purity | > 85 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 350mM NaCl, 1mM DTT, pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | Nucleolar Protein 3 is encoded by NOL3 gene; multiple transcript variants encoding different isoforms have been found for this gene. So far; Nucleolar protein 3 has show to have two Isoforms. Isoform 1 may be involved in RNA splicing.Isoform 2 may inhibit apoptosis.It has been shown to down-regulate the enzyme activities of caspase 2; caspase 8 and tumor protein p53. |
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