Recombinant Human PD-1/PDCD1 Protein (mFc Tag) (PKSH032864)
For research use only.
| Synonyms | CD279, Hsle1, PD-1, PDCD1, Programmed cell death protein 1, SLEB2, hPD-1 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Pro21-Gln167 |
| Accession | Q15116 |
| Calculated Molecular Weight | 42.0 kDa |
| Observed Molecular Weight | 60 kDa |
| Tag | C-mFc |
| Bio-activity | Immobilized Anti-Human PD-1 mAb at 2μg/ml (100 μl/well) can bind Human PD-1-mFc. The ED50 of Human PD-1-mFc is 12.53 ng/ml. |
| Form | Lyophilized powder |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | Programmed cell death protein 1(PDCD1) is a single-pass type I membrane protein and contains 1 Ig-like V-type domain. PD-1 is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 inhibits the T-cell proliferation and production of related cytokines including IL-1; IL-4; IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition; coligation of PDCD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PDCD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance; and thus contributes to the prevention of autoimmune diseases. As a cell surface molecule; PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation; cytokine production; and cytolytic function. |
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