Recombinant Human PSA/KLK3 Protein (aa 25-261, His Tag) (PKSH032949)
For research use only.
| Synonyms | APS, Gamma-Seminoprotein, Hk3, KLK2A1, Kallikrein-3, P-30 Antigen, PSA, Prostate-Specific Antigen, Semenogelase, Seminin |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Ile25-Pro261 |
| Accession | P07288 |
| Calculated Molecular Weight | 27.1 kDa |
| Observed Molecular Weight | 35 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 10mM CaCl2, 150mM NaCl, 0.05% Brij 35, pH 7.5. |
| Reconstitution | Not Applicable |
| Background | KLK3; also known as APS; is short for Prostate-specific antigen. It is a 261 aa. protein which belongs to the peptidase S1 family and Kallikrein subfamily. This protein has 5 isforms produced by alternative splicing. It is a secreted protein and can forms a heterodimer with SERPINA5 which can inhibit its activity. KLK3 is also strongly inhibited by Zn2+; 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins; which are avid zinc binders. KLK3 can hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. |
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