Recombinant Human PSA/KLK3 Protein (His Tag) (PKSH031436)
For research use only.
| Synonyms | APS, Gamma-Seminoprotein, Hk3, KLK2A1, Kallikrein-3, P-30 Antigen, PSA, Prostate-Specific Antigen, Semenogelase, Seminin |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Pro 261 |
| Accession | P07288 |
| Calculated Molecular Weight | 28.3 kDa |
| Observed Molecular Weight | 35 kDa |
| Tag | C-His |
| Bio-activity | Measured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide(Suc-RPY-pNA). The specific activity is > 100 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form) |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | KLK3(Kallikrein 3); also known as Prostate Specific Antigen (PSA); kallikrein-related peptidase 3; Gamma-seminoprotein; is a secreted protein of the glandular kallikrein subfamily of serine proteases.KLK3 contains one peptidase S1 domain.KLK3 is a glycoprotein produced almost exclusively by the prostate gland. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. |
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