Recombinant Human RANTES protein (GST&His Tag) (PDEH100876)
For research use only.
| Synonyms | C-C Motif Chemokine 5, CCL5, D17S136E, EoCP, Eosinophil Chemotactic Cytokine, SCYA5, SIS-Delta, Small-Inducible Cytokine A5, T Cell-Specific Protein P228, T-Cell-Specific Protein RANTES, TCP228 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Pro25-Ser91 |
| Accession | P13501 |
| Calculated Molecular Weight | 32.3 kDa |
| Observed Molecular Weight | 42 kDa |
| Tag | N-GST & C-His |
| Bio-activity | Not validated for activity |
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Endotoxin | < 10 EU/mg of the protein as determined by the LAL method |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
| Background | Human Chemokine (C-C Motif) Ligand 5 (CCL5) plays an active role in recruiting leukocytes into inflammatory sites. CCL5 is secreted by many cell types at inflammatory sites and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5. N-Terminal truncated CCL5/RANTES, Met-RANTES, and amino-oxypentane (AOP)-RANTES exhibit antagonist or partial agonist functions on their receptors. CCL5/RANTES attracts different subtypes of leukocytes into inflamed tissue and intervenes in a wide range of allergic and autoimmune diseases. |
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