Recombinant Human Ribonuclease A/RNASE1 Protein (His Tag) (PKSH033004)
For research use only.
| Synonyms | HP-Rnase, RIB-1, RIB1, RNASE1, RNS1, RNase 1, RNase A, RNase UpI-1, Ribonuclease 1, Ribonuclease A, Ribonuclease Pancreatic |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Lys29-Thr156 |
| Accession | P07998 |
| Calculated Molecular Weight | 15.6 kDa |
| Observed Molecular Weight | 17-33 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 10% Glycerol, pH 7.4. |
| Reconstitution | Not Applicable |
| Background | Ribonuclease Pancreatic is a secreted enzyme that belongs to the pancreatic ribonuclease family. RNASE1 is an endonuclease that cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. RNASE1 prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. RNASE1 acts on single stranded and double stranded RNA. |
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