Recombinant Human Rock-2 Protein(Sumo Tag) (PDEH101129)
For research use only.
| Synonyms | ROCK 2, ROCK2, ROK alpha, ROKalpha, Rho kinase 2, Rho-associated, Rho-associated protein kinase 2, Rock II, Rock2, Rock2m |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Thr1212-Ser1388 |
| Accession | O75116 |
| Calculated Molecular Weight | 32.3 kDa |
| Observed Molecular Weight | 38 kDa |
| Tag | N-Sumo |
| Bio-activity | Not validated for activity |
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Endotoxin | < 10 EU/mg of the protein as determined by the LAL method |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis |
| Background | The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. |
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