Recombinant Human TL1A protein(His Tag) (PKSH034127)
For research use only.
| Synonyms | TNFSF15, VEGI |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Gln 58-Leu 251 |
| Accession | O95150 |
| Calculated Molecular Weight | 23.0 kDa |
| Observed Molecular Weight | 24 kDa |
| Tag | C-His |
| Bio-activity | Measure by its ability to induce TF-1 cells proliferation. The ED50 for this effect is <0.2 ng/mL. |
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from sterile PBS,pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | TL-1A belongs to the TNF superfamily of ligands. It is specially expressed in endothelial cells, and is detected in the placenta, lung, kidney skeletal muscle, pancreas, small intestine and colon. TL-1A inhibits endothelial cell proliferation and angiogenesis. It has been proved to promote NF-KB activation, caspase activity, and apoptosis in responding cell lines. TL-1Acan bind with TNFRSF25/DR3 receptor and a decoy receptor TNFRSF21/DR6. |
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