Recombinant Iba1 Monoclonal Antibody (AN300998L)
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For research use only.
| Verified Samples |
Verified Samples in WB: Mouse spleen Verified Samples in IHC: Human liver tissue, Mouse liver tissue, Human brain |
| Dilution | IHC 1:2000-1:4000, WB 1:1000-1:5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human Iba1 protein |
| Abbre | Iba1 |
| Synonyms | IBA, IRT, PDGF, Protein G, Allograft Inflammatory Factor, AIF-1, Allograft Inflammatory Factor 1, Ionized Calcium-Binding Adapter Molecule 1, IRT1, Protein G1, PDGFA, PDGF1, G1, IBA1, AIF1, AIF 1, AIF1 protein, Allograft inflammatory factor 1 splice variant G, allograft inflammatory factor-1 splice variant Hara-1, balloon angioplasty responsive transcription, BART 1, G1 putative splice variant of allograft inflamatory factor 1, IBA 1, interferon gamma responsive transcript, Interferon responsive transcript 1, interferon responsive transcript factor 1, Ionized calcium binding adapter molecule 1, ionized calcium-binding adapter molecule, IRT 1, Microglia response factor, MRF1 |
| Swissprot | |
| Calculated MW | 17 kDa |
| Observed MW |
17 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cell Biology, Immunology, Neuroscience, Metabolism |
| Clone No. | 12B4 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. |
Other Clones
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Other Formats
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Unconjugated
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