Recombinant IGFBP7 Monoclonal Antibody (AN300063P)
-
-
-
- +1
For research use only.
| Verified Samples |
Verified Samples in WB: HepG2, A431, K562, HeLa in IHC: Human lung cancer in IF: MCF-7 |
| Dilution | WB: 1:1000-1:5000；IHC-P: 1:100-1:200；ICC/IF: 1:100；FC: 1:50-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC-P, ICC/IF, FC |
| Clonality | Recombinant;Monoclonal |
| Immunogen | Recombinant Human IGFBP7 protein |
| Abbre | IGFBP7 |
| Synonyms | MAC, IBP, FSTL, IGF-binding protein, Insulin-like growth factor-binding protein, IGFBPRP, IGFBP-rP, IGFBP7, AGM, FSTL2, IBP-7, IGFBP-7, IGFBP-7v, IGFBPRP1, MAC25, PSF, RAMSVPS, TAF, IGF-binding protein 7, IGFBP-rP1, Insulin-like growth factor-binding protein 7, MAC25 protein, Tumor-derived adhesion factor, Angiomodulin, IBP7, IGF binding protein 7, IGFBP rP1, Insulin like growth factor binding protein 7, PGI2 stimulating factor, PGI2-stimulating factor, Prostacyclin stimulating factor, Prostacyclin-stimulating factor |
| Swissprot | |
| Calculated MW | 29 kDa |
| Observed MW |
29 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Cancer |
| Clone | A1173 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a member of the insulin-like growth factor (IGF)-binding protein (IGFBP) family. IGFBPs bind IGFs with high affinity, and regulate IGF availability in body fluids and tissues and modulate IGF binding to its receptors. This protein binds IGF-I and IGF-II with relatively low affinity, and belongs to a subfamily of low-affinity IGFBPs. It also stimulates prostacyclin production and cell adhesion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and one variant has been associated with retinal arterial macroaneurysm (PMID:21835307). [provided by RefSeq, Dec 2011] |
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.sample_type}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
No files available. Please enter the correct identification code in the search area to query the relevant documents.
| Title | Status | Valid until | Action | |
|---|---|---|---|---|
| {{v.文件名}} | {{v.status_str}} | {{v.timestamp_str}} | Download | / |
*Note：Please download the relevant files within the validity period. After expiration, file preview and download will not be available on the page.
Videos
Resources
Contact with Us
Houston, Texas, 77079, USA
