Recombinant IKK gamma Monoclonal Antibody (AN301804L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, K562, Jurkat Verified Samples in IF: HeLa, NIH/3T3 Verified Samples in FCM: HeLa Verified Samples in IP: HeLa cells extracts |
| Dilution | WB 1:500-1:2000, IF 1:50, FCM 1:50-1:100, IP 1:25-1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB, IF, FCM, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human IKK gamma fragment |
| Abbre | IKK gamma |
| Synonyms | IMD, IPD, AMCBX, IKKAP, ZC2HC, FIP, IKBKG, AMCBX1, FIP-3, FIP3, Fip3p, IKK-gamma, IKKAP1, IKKG, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9, NF-kappa-B essential modulator, FIP 3, gamma, I kappa B kinase gamma, I-kappa-B kinase subunit gamma, IkB kinase associated protein 1, IkB kinase gamma subunit, IkB kinase subunit gamma, IkB kinase-associated protein 1, Incontinentia pigmenti, Inhibitor of kappa light polypeptide gene enhancer in B cells, Inhibitor of nuclear factor kappa B kinase subunit gamma, Inhibitor of nuclear factor kappa-B kinase subunit gamma, kinase gamma, kinase of, NF kappa B essential modifier, NF kappa B essential modulator, NF-kappa-B essential modifier |
| Swissprot | |
| Calculated MW | 48 kDa |
| Observed MW |
48 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cell Biology, Signal Transduction, Epigenetics and Nuclear Signaling, Cardiovascular, Immunology, Cancer |
| Clone No. | A516 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | IKK gamma (IKKγ) is a subunit of a high molecular weight IκB kinase (IKK) complex, which is involved in NF-κB activation. The catalysis of IκB kinase (IKK) complex is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation. Activation of the NF-κB pathway by the T-cell lymphotrophic virus Tax protein or by TNF-α treatment leads to IKKβ-dependent phosphorylation of human IKKγ, primarily at Ser376. In mice, mutation of the orthologous residue (Ser369) to alanine leads to enhanced IKKγ-mediated stimulation of IKKβ kinase activity. |
Other Clones
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