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For research use only.

Verified Samples Verified Samples in WB: Hela, A549, HL-60, U251
Verified Samples in IHC: Human tonsil
Dilution WB 1:500-1:1000,  IHC 1:50-1:100
Isotype IgG
Host Rabbit
Reactivity Human
Applications WB,  IHC-P
Clonality Rabbit Monoclonal
Immunogen A synthetic peptide of human IL-26
Abbre IL 26
Synonyms Protein AK,  AK155,  IL-26,  IL26,  IL 26,  Interleukin 26,  Interleukin-26,  Protein AK155
Swissprot
Calculated MW 20 kDa
Observed MW 20 kDa

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Secreted.
Concentration 300 μg/mL
Buffer 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% stabilizer and 0.05% protective protein.
Purification Method Affinity Purified
Research Areas Immunology
Clone No. R07-3C0
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background IL26 (Interleukin 26) is a Protein Coding gene. Among its related pathways are PEDF Induced Signaling and TGF-Beta Pathway. GO annotations related to this gene include cytokine activity.This gene was identified by its overexpression specifically in herpesvirus samimiri-transformed T cells. The encoded protein is a member of the IL10 family of cytokines. It is a secreted protein and may function as a homodimer. This protein is thought to contribute to the transformed phenotype of T cells after infection by herpesvirus samimiri.
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Unconjugated

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