Recombinant ILF3 Monoclonal Antibody (AN301758L)

Name: Recombinant ILF3 Monoclonal Antibody
Brand: Elabscience
SKU: AN301758L
Price: 249.0 USD
Availability: InStock

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Recombinant ILF3 Monoclonal Antibody - 1

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	100μL	$ 380.00	- +
	50μL	$ 249.00	- +

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Verified Samples	Verified Samples in WB: A431, Neuro-2a, C6, Mouse testis, Rat testis Verified Samples in IHC: Human kidney, Human liver, Human testis, Mouse colon, Mouse liver, Mouse testis, Rat cerebrum, Rat cardiac muscle Verified Samples in IF: HeLa, A431 Verified Samples in FCM: HeLa
Dilution	WB 1:2000-1:10000, IHC 1:100-1:500, IF 1:50, FCM 1:50-1:100
Isotype	IgG, κ
Host	Rabbit
Reactivity	Human, Rat, Mouse
Applications	WB, IHC, IF, FCM
Clonality	Monoclonal;Recombinant
Immunogen	Recombinant human ILF3 fragment
Abbre	ILF3
Synonyms	ILF, MMP, Mpp, DRBP, NF-AT, MPHOSPH, TCP, ILF3, CBTF, DRBF, DRBP76, MMP4, MPHOSPH4, MPP4, NF-AT-90, NF110, NF110b, NF90, NF90a, NF90b, NFAR, NFAR-1, NFAR2, TCP110, TCP80, Double stranded RNA binding protein 76, Double-stranded RNA-binding protein 76, Interleukin enhancer binding factor 3, Interleukin enhancer-binding factor 3, M phase phosphoprotein 4, M-phase phosphoprotein 4, NF AT 90, Nuclear factor associated with dsRNA, Nuclear factor of activated T cells 90 kDa, Nuclear factor of activated T-cells 90 kDa, Translational control protein 80
Swissprot	Q12906
Calculated MW	95 kDa
Observed MW	90,110 kDa The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization	Nucleus
Concentration	1 mg/mL
Buffer	PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Purification Method	Protein A purified
Research Areas	Signal Transduction, Epigenetics and Nuclear Signaling
Clone No.	A466
Conjugation	Unconjugated
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping	Ice bag
background	Interleukin enhancer-binding factor 3 (ILF3) is a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein (NF90, ILF3) forms a heterodimer with a 45 kDa transcription factor (NF45, ILF2) required for T-cell expression of interleukin 2. This complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms. ILF3 exists main two isoforms NFAR-1 (90 kDa) and -2 (110 kDa). Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response.