Recombinant JAK3 Monoclonal Antibody (AN300939L)
For research use only.
| Verified Samples | Verified Samples in WB: KARPAS-299 |
| Dilution | WB 1:1000-1:5000, |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human JAK3 protein |
| Abbre | JAK3 |
| Synonyms | JAK, JAK3, EC 2.7.10.2, JAK 3, JAK L, JAK-3, JAK3 HUMAN, JAK3_HUMAN, JAKL, Janus kinase 3, Janus kinase 3 (a protein tyrosine kinase, Janus Kinase3, L JAK, Leukocyte janus kinase, leukocyte), LJAK, L-JAK, Protein tyrosine kinase leukocyte, Tyrosine protein kinase JAK3, Tyrosine-protein kinase JAK3 |
| Swissprot | |
| Calculated MW | 125 kDa |
| Observed MW |
125 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Epigenetics and Nuclear Signaling, Cancer |
| Clone No. | 7A13 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease). |
Other Clones
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Unconjugated
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