Recombinant Kappa light chain Monoclonal Antibody (AN301847L)
For research use only.
| Verified Samples |
Verified Samples in WB: Human plasma Verified Samples in IHC: Human tonsil |
| Dilution | WB 1:2000-1:10000, IHC 1:200-1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human Kappa light chain fragment |
| Abbre | Kappa light chain |
| Synonyms | IGKC |
| Swissprot | |
| Calculated MW | 25 kDa |
| Observed MW |
25 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane, Secreted |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Immunology |
| Clone No. | A559 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Antibody production is a response to foreign molecules invading the body. Antibodies exist as one or more Y-shaped monomers consisting of four polypeptide chains. Each Y-shaped monomer contains two identical heavy chains and two identical light chains, which differ in sequence and length. The top of the Y-shaped monomer contains a variable region that can specifically bind tightly to an epitope on an antigen. The light chains of antibodies can be classified as either kappa (κ) or lambda (λ) types based on minor differences in their polypeptide sequences. |
Other Clones
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Unconjugated
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