Recombinant Keratin 16 Monoclonal Antibody (AN301575L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HeLa Verified Samples in IHC: Human esophagus, Human tonsil Verified Samples in IF: A431 Verified Samples in IP: HeLa cells extracts |
| Dilution | WB 1:1000-1:5000, IHC 1:200-1:1000, IF 1:50, IP 1:25-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC, IF, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human Keratin 16 fragment |
| Abbre | Keratin 16 |
| Synonyms | KRT, KRT16, CK16, FNEPPK, K16, K1CP, KRT16A, NEPPK, PC1, CK 16, CK-16, Cytokeratin16, Cytokeratin-16, Focal non epidermolytic palmoplantar keratoderma, K 16, K1C16, Keratin, Keratin 1 type I, Keratin 16, Keratin type I cytoskeletal 16, Keratin16, Keratin-16, KRT 16, type I cytoskeletal 16 |
| Swissprot | |
| Calculated MW | 51 kDa |
| Observed MW |
51 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane, cytoplasm |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction |
| Clone No. | A274 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance. |
Other Clones
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Other Formats
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Unconjugated
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