Recombinant KRAS Monoclonal Antibody (AN301583L)
For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, HCT-116, SW480, Rat brain, Mouse brain Verified Samples in IF: HL-60 Verified Samples in IP: HeLa cells extracts |
| Dilution | WB 1:500-1:2000, IF 1:50, IP 1:25-1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IF, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human KRAS fragment |
| Abbre | KRAS |
| Synonyms | CFC, K-Ras, KRAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RALD, RASK2, c-Ki-ras2, GTPase KRas, RASK, c-Ki-ras, K-Ras 2, c Ki ras2, c Kirsten ras protein, Cellular c Ki ras2 proto oncogene, Cellular transforming proto oncogene, cK Ras, GTPase, K RAS p21 protein, K RAS2A, K RAS2B, K RAS4A, K RAS4B, KI RAS, KIRSTEN MURINE SARCOMA VIRUS 2, Kirsten rat sarcoma 2 viral (v Ki ras2) oncogene homolog, Kirsten rat sarcoma viral oncogene homolog, KRAS proto oncogene, N-terminally processed, Oncogene KRAS2, p21ras, PR310 c K ras oncogene, PR310 cK ras oncogene, Transforming protein p21, v Ki ras2 Kirsten rat sarcoma 2 viral oncogene homolog, v Ki ras2 Kirsten rat sarcoma viral oncogene homolog |
| Swissprot | |
| Calculated MW | 22 kDa |
| Observed MW |
21 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Epigenetics and Nuclear Signaling, Cancer |
| Clone No. | A282 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | KRAS, a Ras protein, binds GDP/GTP and possesses intrinsic GTPase activity; It plays an important role in the regulation of cell proliferation and in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner. |
Other Clones
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Unconjugated
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