Recombinant Lactate Dehydrogenase Isoenzyme V Monoclonal Antibody (AN301095L)
For research use only.
| Verified Samples |
Verified Samples in WB: A431 Verified Samples in IHC: Human appendix tissue, Mouse heart tissue |
| Dilution | IHC 1:200-1000, WB 1:500-2000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human Lactate Dehydrogenase Isoenzyme V protein |
| Abbre | Lactate Dehydrogenase Isoenzyme V |
| Synonyms | GSD, l7R, PIG19 Proliferation-inducing gene, PIG, GSD11, HEL-S-133P, LDHM, PIG19, LDHA, Cell proliferation-inducing gene 19 protein, L lactate dehydrogenase A chain, l7R2, Lactate dehydrogenase A, LDH A, LDH M, LDH muscle subunit, LDH1, LDH5, LDH-A, LDH-M, L-Lactate Dehydrogenase A Chain, PIG 19, PIG19 Proliferation-inducing gene 19, Renal carcinoma antigen NY-REN-59, GSD11, L lactate dehydrogenase A chain, l7R2, LDH M, LDH muscle subunit, LDH1, PIG19 Proliferation-inducing gene 19, LDHA |
| Swissprot | |
| Calculated MW | 36 kDa |
| Observed MW |
36 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Cancer, Metabolism |
| Clone No. | 6A4 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. |
Other Clones
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Unconjugated
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