Recombinant LC3B Monoclonal Antibody (AN301035L)
For research use only.
| Verified Samples |
Verified Samples in WB: Hela Verified Samples in IHC: Human brain tissue, Mouse brain tissue |
| Dilution | IHC 1:200-1:1000, WB 1:1000-1:5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human LC3B protein |
| Abbre | LC3B |
| Synonyms | MAP1A/1BLC, MAP1 light chain 3-like protein, MAP1ALC, MAP1LC3B, ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a, microtubule associated protein 1 light chain 3 beta, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, LC3A/LC3B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, Microtubule-associated protein 1 light chain 3 beta, MAP1ALC3, Autophagy related protein LC3 A, Autophagy related ubiquitin like modifier LC3 A, Autophagy related ubiquitin like modifier LC3 B, LC3, LC3II, MAP, MAP1 light chain 3 like protein 1, MAP1 light chain 3 like protein 2, MAP1A/1B light chain 3 A, MAP1LC3B a, Microtubule-associated proteins 1A/1B light chain 3B, MLP3B |
| Swissprot | |
| Calculated MW | 14 kDa,16 kDa |
| Observed MW |
14 kDa,16 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Neuroscience, Cardiovascular, Cancer, Metabolism |
| Clone No. | 5A10 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. |
Other Clones
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Other Formats
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Unconjugated
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