Recombinant Leukotriene A4 Hydrolase/LTA4H Monoclonal Antibody (AN300111P)
For research use only.
| Verified Samples |
Verified Samples in WB: K562, C6, 3T3, Hela in IHC: Human tonsil |
| Dilution | WB: 1:2000-1:20000；IHC-P: 1:200-1:500；FC: 1:100-1:1000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-P, FC |
| Clonality | Recombinant;Monoclonal |
| Immunogen | Recombinant Human Leukotriene A4 Hydrolase / LTA4H protein |
| Abbre | LTA4H |
| Synonyms | LTA, LKHA, LTA4H, FLJ17564, Leukotriene A(4) hydrolase, Leukotriene A4 hydrolase, Leukotriene A-4 hydrolase, LKHA4, LTA4 hydrolase, LTA-4 hydrolase, LTA4 |
| Swissprot | |
| Calculated MW | 69 kDa |
| Observed MW |
69 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Immunology, Cancer, Metabolism |
| Clone | A1225 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene is an enzyme that contains both hydrolase and aminopeptidase activities. The hydrolase activity is used in the final step of the biosynthesis of leukotriene B4, a proinflammatory mediator. The aminopeptidase activity has been shown to degrade proline-glycine-proline (PGP), a neutrophil chemoattractant and biomarker for chronic obstructive pulmonary disease (COPD). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] |
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