Recombinant Lysozyme Monoclonal Antibody (AN301868L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HeLa (Negative control), THP-1 Verified Samples in IHC: Human spleen, Human tonsil Verified Samples in IP: THP-1 cells extracts |
| Dilution | WB 1:500-1:2000, IHC 1:200-1:1000, IP 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human Lysozyme fragment |
| Abbre | Lysozyme |
| Synonyms | LYZF, LYZ, LYZF1, LZM, lysozyme, 4-beta-N-acetylmuramidase C, Lysozyme C, 1, 4-beta-N-acetylmuramidase C, muramidase, N-acetylmuramide glycanhydrolase |
| Swissprot | |
| Calculated MW | 17 kDa |
| Observed MW |
13 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Immunology, Cardiovascular, Tags & Cell Markers |
| Clone No. | A580 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Lysozyme belongs to the glycosyl hydrolase 22 family. It catalyzes the hydrolysis of the bacterial cell wall beta glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. In tissues and body fluids this protein is associated with the monocyte-macrophage system and enhance the activity of immunoagents. |
Other Clones
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Unconjugated
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