Recombinant MCM7 Monoclonal Antibody (AN301748L)

Name: Recombinant MCM7 Monoclonal Antibody
Brand: Elabscience
SKU: AN301748L
Price: 249.0 USD
Availability: InStock

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Recombinant MCM7 Monoclonal Antibody - 1

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	100μL	$ 380.00	- +
	50μL	$ 249.00	- +

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Verified Samples	Verified Samples in WB: HeLa Verified Samples in IHC: Human lung adenocarcinoma, Human tonsil Verified Samples in IF: Cos-7, MCF-7 Verified Samples in FCM: HeLa, MCF-7
Dilution	WB 1:2000-1:10000, IHC 1:50-1:100, IF 1:50, FCM 1:50-1:100
Isotype	IgG, κ
Host	Rabbit
Reactivity	Human, African green monkey
Applications	WB, IHC, IF, FCM
Clonality	Monoclonal;Recombinant
Immunogen	Recombinant human MCM7 fragment
Abbre	MCM7
Synonyms	CDC, PNAS, P1CDC, PPP1R, P1.1-MCM, MCM, MCM7, CDC47, MCM2, P1.1-MCM3, P1CDC47, P85MCM, PNAS146, PPP1R104, formerly, homolog of, regulatory subunit 104, S. cerevisiae, CDABP0042, CDC 47, CDC47 homolog, DNA replication licensing factor MCM7, Homolog of S. cerevisiae Cdc47, MCM 2, MCM 7, MCM7 minichromosome maintenance deficient 7, MCM7_HUMAN, Minichromosome maintainence, Minichromosome Maintainence 7, Minichromosome maintenance complex component 7, Minichromosome maintenance deficient 7, Minichromosome maintenance protein 7, P1.1 MCM3, PNAS 146, Protein phosphatase 1
Swissprot	P33993
Calculated MW	81 kDa
Observed MW	81 kDa Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization	Nucleus
Concentration	1 mg/mL
Buffer	PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Purification Method	Protein A purified
Research Areas	Epigenetics and Nuclear Signaling
Clone No.	A456
Conjugation	Unconjugated
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping	Ice bag
background	DNA replication licensing factor MCM7 acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for once per cell cycle DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. MCM7 is also required for S-phase checkpoint activation upon UV-induced damage.