Recombinant MFN1 Monoclonal Antibody (AN300620L)
For research use only.
| Verified Samples | Verified Samples in WB: C2C12 |
| Dilution | WB 1:2000-1:10000, |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human MFN1 protein |
| Abbre | MFN1 |
| Synonyms | MFN, MFN1, hfzo1, hfzo2, Fzo homolog, MFN 1, Mitochondrial transmembrane GTPase Fzo 1, Mitochondrial transmembrane GTPase FZO 2, Mitochondrial transmembrane GTPase FZO1B, Mitofusin 1, Mitofusin1, Mitofusin-1, MS996, Putative transmembrane GTPase, Transmembrane GTPase MFN1 |
| Swissprot | |
| Calculated MW | 84 kDa |
| Observed MW |
84 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Mitochondrion outer membrane, Multi-pass membrane protein, Cytoplasm. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Neuroscience, Tags & Cell Markers, Signal Transduction, Metabolism, Cell Biology, Cancer |
| Clone No. | 5A7 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting. |
Other Clones
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Unconjugated
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