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Recombinant MMP13 Monoclonal Antibody - 1
  • Recombinant MMP13 Monoclonal Antibody - 1
  • Recombinant MMP13 Monoclonal Antibody - 2
All Size Price Qty
100μL $ 320.00
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50μL $ 211.00
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For research use only.

Verified Samples Verified Samples in WB: Hela
Dilution WB 1:500-2000
Isotype IgG,κ
Host Rabbit
Reactivity Human,  Mouse
Applications WB
Clonality Monoclonal;Recombinant
Immunogen Recombinant Human MMP13 protein
Abbre MMP13
Synonyms MANDP,  Pro-MMP,  CLG,  MMP,  MMP13,  CLG3,  MANDP1,  MDST,  MMP-13,  CLG 3,  Collagenase3,  Matrix metallopeptidase 13 (collagenase 3),  Matrix Metalloproteinase 13,  MMP 13,  Pro-MMP-13,  Collagenase 3,  Matrix metalloproteinase-13,  MMP-13
Swissprot
Calculated MW 60 kDa
Observed MW 60 kDa

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Secreted
Tissue Specificity Breast carcinoma
Concentration 0.2 mg/mL
Buffer PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Purification Method Protein A
Research Areas Cardiovascular,  Signal Transduction,  Cancer,  Cell Biology,  Kits,  Lysates,  Other
Clone No. 8D6
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Mutations in this gene are associated with metaphyseal anadysplasia. This gene is part of a cluster of MMP genes on chromosome 11.
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Unconjugated

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