Recombinant Mouse CCL6/C10 Protein(Trx Tag) (PDEM100152)
For research use only.
| Synonyms | Scya, Chemokine C C motif Ligand, Small Inducible Cytokine A, chemokine (C-C motif) ligand, CCL 6, chemokine (C-C motif) ligand 6, Chemokine C C motif Ligand 6, MRP1, MRP-1, Small Inducible Cytokine A6, C10, Scya6, Ccl6, C10, CCL 6, chemokine (C-C motif) ligand 6, Chemokine C C motif Ligand 6, MRP1, SCYA6, Small Inducible Cytokine A6 |
| Species | Mouse |
| Expression Host | E.coli |
| Sequence | Gly22-Ala116 |
| Accession | P27784 |
| Calculated Molecular Weight | 30.3 kDa |
| Observed Molecular Weight | 32 kDa |
| Tag | N-Trx |
| Bio-activity | Not validated for activity |
| Purity | > 90% as determined by reducing SDS-PAGE. |
| Endotoxin | < 10 EU/mg of the protein as determined by the LAL method |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
| Background | In mice , CCL6/C10 is expressed in cells from neutrophil and macrophage lineages , and can be greatly induced under conditions suitable for myeloid cell differentiation. It is highly expressed in bone marrow cultures that have been stimulated with the cytokine GM-CSF. Some low levels of gene expression also occur in certain cell lines of myeloid origin (e.g. the immature myeloid cell lines DA3 and 32D cl3 , and the macrophage cell line P388D) that can also be greatly induced in culture with GM-CSF. However , in activated T cell lines , expression of CCL6/C10 is greatly reduced.CCL6/C10 can also be induced in the Mouse lung by the cytokine interleukin 13 |
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