Recombinant Mouse CD34 Protein (His Tag) (PKSM040592)
For research use only.
| Synonyms | AU040960 |
| Species | Mouse |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Thr 287 |
| Accession | NP_001104529.1 |
| Calculated Molecular Weight | 28.6 kDa |
| Observed Molecular Weight | 50-60 kDa |
| Tag | C-His |
| Bio-activity | Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line. When 4 x 104 cells/well are added to mouse CD34 coated plates (0.8 μg/ml, 100 μl/well), approximately > 30 % will adhere after one hour at 37 ℃. |
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | Cluster of Differentiation 34 (CD34) is a member of a family of single-pass transmembrane sialomucin proteins, and may function as a cell-cell adhesion factor. CD34 protein is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. It has been widely used as a stem and progenitor cell marker, and clinical CD34+ stem cell transplantation (CD34+SCT) has been performed for tumor purging. CD34 monoclonal antibodies are widely used to identify and isolate hemopoietic progenitors and to classify acute and chronic leukemias. |
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