Recombinant Mouse Csf3 Protein(Trx Tag) (PDEM100115)
For research use only.
| Synonyms | CSF, TNFSF, C17orf, C17orf33, CD254, CSF3OS, Filgrastim, GCSF, G-CSF, Granulocyte colony-stimulating factor, Lenograstim, ODF (Osteoclast Differentiation Factor), OPGL, Pluripoietin, soluble Receptor Activator of NF-kB Ligand, sRNAK Ligand, TNFSF11, TRANCE (TNF-Related Activation-induced Cytokine), Csfg, Csf3 |
| Species | Mouse |
| Expression Host | E.coli |
| Sequence | Val31-Ala208 |
| Accession | P09920 |
| Calculated Molecular Weight | 39.6 kDa |
| Observed Molecular Weight | 35 kDa |
| Tag | N-Trx |
| Bio-activity | Not validated for activity |
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Endotoxin | < 10 EU/mg of the protein as determined by the LAL method |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis |
| Background | Granulocyte colony-stimulating factor (G-CSF) is a growth factor and an essential cytokine which belongs to the IL-6 superfamily. Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. G-CSF binding to its receptor G-CSF-R which belongs to the cytokine receptor type I family depends on the interaction of alpha-helical motifs of the former and two fibronectin type III as well as an immunoglobulin-like domain of the latter. G-CSF is a cytokine that have been demonstrated to improve cardiac function and perfusion in myocardial infarction. And it was initially evaluated as a stem cell mobilizer and erythropoietin as a cytoprotective agent. |
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