Recombinant Mouse IL-27 EBI3 protein(His Tag) (PKSM041472)
For research use only.
| Synonyms | EBI, Lymphopoietin 1(LP-1), pre-B-cell factor, Il27b, Ebi3 |
| Species | Mouse |
| Expression Host | E.coli |
| Sequence | Ala 23-Pro 228 |
| Accession | O35228 |
| Calculated Molecular Weight | 23.8 kDa |
| Observed Molecular Weight | 25 kDa |
| Tag | C-His |
| Bio-activity | Measure by its ability to protect HepG2 cells infected with encephalomyocarditis (EMC) virus.The ED50 for this effect is <5 ng/mL. |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | Possesses antiviral functions and induces the type I intereferon response during influenza infection. Signals through two receptor complexes IL20RA/IL20RB or IL20RB/IL22RA1. In turn, stimulates the JAK1-STAT3 and MAPK pathways and promotes the secretion of pro-inflammatory mediators including IL8 and MMP1 (By similarity). |
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