Recombinant Mouse IL-5 Protein(Fc Tag) (PDMM100077)
For research use only.
| Synonyms | B-cell growth factor II (BCGF-II), Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, T-cell replacing factor (TRF), Il5 |
| Species | Mouse |
| Expression Host | HEK293 Cells |
| Sequence | Met1-Gly133 |
| Accession | P04401 |
| Calculated Molecular Weight | 39.5 kDa |
| Observed Molecular Weight | 40-60 kDa |
| Tag | C-Fc |
| Bio-activity | Not validated for activity |
| Form | Lyophilized powder |
| Purity | > 90% as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU/mg of the protein as determined by the LAL method |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
| Background | IL-5 (Interleukin-5) is a secreted cytokine that is expressed as a covalent dimer. It is produced by activated Th2 cells, eosinophils, mast cells, and iNKT cells and promotes eosinophilic and basophilic differentiation and activation. IL-5 signals through a receptor complex containing IL-5 R alpha and the Common beta Chain/CD131 on eosinophils and basophils. Naturally occurring soluble forms of IL-5 R alpha retain the ability to bind IL-5 and limit its bioavailability. |
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