Recombinant Mouse INS protein (GST Tag) (PDEM100243)
For research use only.
| Synonyms | IDDM, IRDN, Ins-2, Insulin B chain, Proinsulin, ins |
| Species | Mouse |
| Expression Host | E.coli |
| Sequence | Phe25-Asn110 |
| Accession | P01326 |
| Calculated Molecular Weight | 34.4 kDa |
| Observed Molecular Weight | 30 kDa |
| Tag | N-GST |
| Bio-activity | Not validated for activity |
| Form | Lyophilized powder |
| Purity | > 95% as determined by reducing SDS-PAGE. |
| Endotoxin | < 10 EU/mg of the protein as determined by the LAL method |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
| Background | Insulin is a disulfide-linked heterodimeric protein secreted by the pancreatic Islets of Langerhans. Mature insulin is generated by the proteolytic removal of a peptide from proinsulin. It is involved in the regulation of glucose metabolism through interactions with the Insulin Receptor. |
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