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Recombinant Mouse Kallikrein 1/KLK1 Protein (His Tag) (PKSM040442)

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50μg $ 760.00
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For research use only.

Synonyms Glandular kallikrein K1, KAL-B, Renal kallikrein, Tissue kallikrein-6, mGK-6
Species Mouse
Expression Host HEK293 Cells
Sequence Met1-Asp261
Accession P15947
Calculated Molecular Weight 28.3 kDa
Observed Molecular Weight 36 kDa
Tag C-His
Bio-activity Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin(PFR-AMC). The specific activity is > 6, 000 pmol/min/μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
Purity > 90 % as determined by reducing SDS-PAGE.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile 25 mM Tris, 5 mM CaCl2, 0.15 M NaCl, pH 7.4
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.
Background Human tissue kallikrein (KLK1) is a serine protease, component of the KKS that has been demonstrated to exert pleiotropic beneficial effects in protection from tissue injury through its antiinflammatory, antiapoptotic, antifibrotic and antioxidative actions.Polymorphism of the human tissue kallikrein 1 (KLK1) A1789G gene is associated with susceptibility to hypertension.
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