Recombinant Mouse TRAIL R2/TNFRSF10B Protein (His Tag) (PKSM040694)
For research use only.
| Synonyms | CD262, DR5, Death Receptor 5, KILLER, TNF-Related Apoptosis-Inducing Ligand Receptor 2, TNFRSF10B, TRAIL Receptor 2, TRAIL-R2, TRAILR2, TRICK2, Tumor Necrosis Factor Receptor Superfamily Member 10B, ZTNFR9 |
| Species | Mouse |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Ser 177 |
| Accession | NP_064671.2 |
| Calculated Molecular Weight | 15 kDa |
| Observed Molecular Weight | 25-35 kDa |
| Tag | C-His |
| Bio-activity | 1. Immobilized mouse TNFRSF10B-His at 10 μg/ml (100 μl/well) can bind biotinylated human TNFSF10, The EC50 of biotinylated human TNFSF10 is 0.16-0.38 μg/ml. 2. Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L‑929 mouse fibroblast cells treated with TRAIL. The ED50 for this effect is typically 0.5-2 μg/mL in the presence of 20 ng/ml Recombinant Human TRAIL/TNFSF10. |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | Tumor necrosis factor receptor superfamily, member 10b, official symbol TNFRSF10B, also known as Death receptor 5, CD262, TNF-related apoptosis-inducing ligand receptor 2 (TRAIL R2), is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. TRAIL R2/CD262/TNFRSF10B was purified independently as the only receptor for TRAIL detectable on the surface of two different human cell lines that undergo apoptosis upon stimulation with TRAIL. TRAIL R2/CD262/TNFRSF10B contains two extracellular cysteine-rich repeats, typical for TNF receptor (TNFR) family members, and a cytoplasmic death domain. TRAIL R2/CD262/TNFRSF10B mediates apoptosis via the intracellular adaptor molecule FADD/MORT1. TRAIL receptors can signal both death and gene transcription, functions reminiscent of those of TNFR1 and TRAMP, two other members of the death receptor family. Defects in TRAIL R2/CD262/TNFRSF10B may be a cause of head and neck squamous cell carcinomas (HNSCC) also known as squamous cell carcinoma of the head and neck. |
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