Recombinant MPTX Monoclonal Antibody (AN301603L)
For research use only.
| Verified Samples |
Verified Samples in WB: Mouse colon, Mouse small intestine Verified Samples in IHC: Mouse small intestine |
| Dilution | WB 1:500-1:2000, IHC 1:2000-1:5000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Mouse |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human MPTX fragment |
| Abbre | MPTX |
| Synonyms | Mptx, Mptx1 |
| Swissprot | |
| Calculated MW | 25 kDa |
| Observed MW |
27 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Extracellular region, secreted |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Clone No. | A306 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Mptx expression is related to colonic cell turnover, and that Mptx might be a marker for diet-modulated mucosal integrity. Mptx expression is restricted to the intestine, and occurs predominantly in the colon. Mptx mRNA is expressed in rat and mouse colon, but not in human colon. |
Other Clones
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Other Formats
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Unconjugated
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