Recombinant MSH6/GTBP Monoclonal Antibody (AN300129P)
For research use only.
| Verified Samples |
Verified Samples in WB: K562, C6, 3T3, Hela in IHC: Human tonsil in IF: HeLa |
| Dilution | WB: 1:1000-1:5000；IHC-P: 1:200-1:500；ICC/IF: 1:200-1:500 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC-P, ICC/IF |
| Clonality | Recombinant;Monoclonal |
| Immunogen | A synthetic peptide corresponding to the center region of the Human MSH6 / GTBP |
| Abbre | MSH6 |
| Synonyms | MSH, mutS homolog, HNPCC, MSH6, GTBP, GTMBP, HNPCC5, HSAP, p160, mutS homolog 6, GTBP, GTMBP, HNPCC5, HSAP, mutS homolog 6, p160 |
| Swissprot | |
| Calculated MW | 163 kDa |
| Observed MW |
163 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Epigenetics and Nuclear Signaling |
| Clone | A1243 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a member of the DNA mismatch repair MutS family. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein heterodimerizes with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene may be associated with hereditary nonpolyposis colon cancer, colorectal cancer, and endometrial cancer. Transcripts variants encoding different isoforms have been described. [provided by RefSeq, Jul 2013] |
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