Recombinant MUC2 Monoclonal Antibody (AN300695L)
For research use only.
| Verified Samples | Verified Samples in WB: Mouse colon |
| Dilution | WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human MUC2 protein |
| Abbre | MUC2 |
| Synonyms | MUC, MUC2, MLP, MUC-2, SMUC, mucin-2, Intestinal mucin 2, MUC 2, Mucin 2 intestinal, Mucin 2 intestinal/tracheal, Mucin 2 oligomeric mucus/gel forming, Mucin 2 precursor, Mucin 2 tracheal, Mucin like protein, Mucin2, Mucin 2, Intestinal mucin-2, Intestinal mucin 2, Intestinal mucin-2, MLP, MUC 2, MUC-2, Muc2, MUC2, Mucin 2, Mucin 2 intestinal, Mucin 2 intestinal/tracheal, Mucin 2 oligomeric mucus/gel forming, Mucin 2 precursor, Mucin 2 tracheal, Mucin like protein, Mucin-2, Mucin2, SMUC |
| Swissprot | |
| Calculated MW | 551 kDa |
| Observed MW |
140-170 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Tags & Cell Markers, Signal Transduction, Cancer |
| Clone No. | 10F11 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. |
Other Clones
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Unconjugated
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