Recombinant N Cadherin Monoclonal Antibody (AN300983L)
For research use only.
| Verified Samples |
Verified Samples in WB: Mouse brain Verified Samples in IHC: Human kidney carcinoma tissue, Rat liver tissue |
| Dilution | IHC 1:500-1:2000, WB 1:1000-1:5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human N Cadherin protein |
| Abbre | N Cadherin |
| Synonyms | CDH, CDw, Cadherin 2 Type, cadherin, CD325, CDHN, CDw325, NCAD, N Cadherin, CDH2, Cadherin 2 Type 1, cadherin-2, N-Cadherin, CDH2 CDw325, cadherin-2, CD325, CDHN, CDw325, NCAD, CD325, CDHN, CDw325, NCAD, N Cadherin, CDH2, N-cadherin |
| Swissprot | |
| Calculated MW | 125 kDa |
| Observed MW |
130 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Signal Transduction, Stem Cells, Cancer, Tags & Cell Markers |
| Clone No. | 11A6 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. |
Other Clones
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Other Formats
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Unconjugated
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