Recombinant NAP1L1 Monoclonal Antibody (AN302039L)
For research use only.
| Verified Samples |
Verified Samples in WB: HeLa,?293T, A431, Jurkat Verified Samples in IHC: Human colon, Human kidney |
| Dilution | WB 1:2000, IHC 1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Peptide. This information is proprietary to PTMab. |
| Abbre | NAP1L1 |
| Synonyms | NAP, NAP1L1, NAP1, NAP1L, NRP, FLJ16112, hNRP, HSP22 like protein interacting protein, MGC23410, MGC8688, NAP 1, NAP 1 related protein, NAP 1L, NAP1 L1, NAP1 related protein, NAP1L1 protein, NAP-1-related protein, NP1L1, Nucleosome assembly protein 1 like 1, Nucleosome assembly protein 1-like 1, Nucleosome assembly protein I-related protein |
| Swissprot | |
| Calculated MW | 45 kDa |
| Observed MW |
50, 55 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Epigenetics and Nuclear Signaling |
| Clone No. | A759 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | NAP1L1 (Nucleosome assembly protein 1-like 1; NAP1-Related Protein, NRP) is a member of the NAP family of histone chaperones. Histone chaperones are involved in chromatin assembly following DNA replication and may play a role in modulating chromatin formation and contribute to the regulation of cell proliferation. |
Other Clones
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Other Formats
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Unconjugated
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