Recombinant NCAM Monoclonal Antibody (AN300345P)
For research use only.
| Verified Samples | Verified Samples in IF:SHSY5Y |
| Dilution | WB 1:1000, IHC-P 1:50-1:100, ICC/IF 1:200-1:1000, IP 1:20 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | ICC/IF |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human NCAM Protein |
| Abbre | NCAM1 |
| Synonyms | MSK, NKH, Leu, N-CAM, NCAM1 /CD, CD56, MSK39, NCAM, NCAM1, NCAM1 /CD56, NKH1, CD56 antigen, Leu-19, NCAM-1, N-CAM-1, neural cell adhesion molecule, Neural cell adhesion molecule 1, Leu-19, NKH-1, antigen MSK39 identified by monoclonal 5.1H11, antigen recognized by monoclonal 5.1H11, cell adhesion molecule, MSK 39, NCAM 1, NCAM C, NCAMC, neural, neural cell adhesion molecule, Neural cell adhesion molecule NCAM, OTTHUMP00000235666 |
| Swissprot | |
| Calculated MW | 95 kDa |
| Observed MW |
120-220 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane,Secreted |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Affinity Purification |
| Research Areas | Neuroscience, Stem Cells |
| Clone | B267 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein plays a role in the development of the nervous system by regulating neurogenesis, neurite outgrowth, and cell migration. This protein is also involved in the expansion of T lymphocytes, B lymphocytes and natural killer (NK) cells which play an important role in immune surveillance. This protein plays a role in signal transduction by interacting with fibroblast growth factor receptors, N-cadherin and other components of the extracellular matrix and by triggering signalling cascades involving FYN-focal adhesion kinase (FAK), mitogen-activated protein kinase (MAPK), and phosphatidylinositol 3-kinase (PI3K). One prominent isoform of this gene, cell surface molecule CD56, plays a role in several myeloproliferative disorders such as acute myeloid leukemia and differential expression of this gene is associated with differential disease progression. For example, increased expression of CD56 is correlated with lower survival in acute myeloid leukemia patients whereas increased severity of COVID-19 is correlated with decreased abundance of CD56-expressing NK cells in peripheral blood. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Aug 2020] |
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