Recombinant NCAM1/CD56 Monoclonal Antibody (AN301702L)
For research use only.
| Verified Samples |
Verified Samples in WB: SH-SY5Y, Mouse cerebellum, Rat cerebellum Verified Samples in FCM: SH-SY5Y Verified Samples in IP: SH-SY5Y cells extracts |
| Dilution | WB 1:500-1:2000, FCM 1:50-1:100, IP 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, FCM, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human NCAM1/CD56 fragment |
| Abbre | NCAM1/CD56 |
| Synonyms | MSK, NKH, Leu, N-CAM, NCAM1 /CD, CD56, MSK39, NCAM, NCAM1, NCAM1 /CD56, NKH1, CD56 antigen, Leu-19, NCAM-1, N-CAM-1, neural cell adhesion molecule, Neural cell adhesion molecule 1, Leu-19, NKH-1, antigen MSK39 identified by monoclonal 5.1H11, antigen recognized by monoclonal 5.1H11, cell adhesion molecule, MSK 39, NCAM 1, NCAM C, NCAMC, neural, neural cell adhesion molecule, Neural cell adhesion molecule NCAM, OTTHUMP00000235666 |
| Swissprot | |
| Calculated MW | 95 kDa |
| Observed MW |
140-180 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane, Secreted |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Neuroscience, Stem Cells |
| Clone No. | A410 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | In human hematopoiesis, CD56, also known as neural cell adhesion molecule 1 (NCAM1) is highly expressed in natural killer (NK) cells. In addition, NCAM1 expression was found in rare subsets of T and B lymphocytes, dendritic cells, and neural or mesenchymal stem cells. NCAM1 belongs to the immunoglobulin superfamily of adhesion molecules. NCAM1 plays an important role in the regulation of neurogenesis, neurite outgrowth, proliferation, and cell migration. |
Other Clones
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Other Formats
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Unconjugated
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