Recombinant NCK1 Monoclonal Antibody (AN302021L)
For research use only.
| Verified Samples | Verified Samples in WB: HeLa,?293T, Mouse brain, Rat brain |
| Dilution | WB 1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Peptide. This information is proprietary to PTMab. |
| Abbre | NCK1 |
| Synonyms | NCK adaptor Protein, Cytoplasmic Protein NCK, NCK1, NCK, NCKalpha, nck-1, Cytoplasmic Protein NCK1, NCK adaptor Protein 1, SH2/SH3 Adaptor Protein NCK-Alpha, Melanoma Nck protein, MGC12668, NCK 1, NCK alpha, NCK tyrosine kinase, Non catalytic region of tyrosine kinase, SH2/SH3 adaptor protein NCK alpha |
| Swissprot | |
| Calculated MW | 43 kDa |
| Observed MW |
50 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus, Endoplasmic reticulum |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Epigenetics and Nuclear Signaling, Cancer |
| Clone No. | A741 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | NCK(also known as NCK1 or NCKα) is a broadly expressed oncogenic adapter protein consisting of three SH3 domains and one SH2 domain. NCK1 becomes phosphorylated upon activation of variety of cell surface receptors and is involved in actin cytoskeletal organization induced by many stimuli. NCK2 (also known as NCKβ), a homolog of NCK1, has an overlapping expression pattern and redundant functions with NCK1. |
Other Clones
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Unconjugated
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