Recombinant p27 KIP 1 Monoclonal Antibody (AN301021L)
For research use only.
| Verified Samples |
Verified Samples in WB: Hela Verified Samples in IHC: Human brain tissue, Mouse brain tissue |
| Dilution | IHC 1:200-1:1000, WB 1:1000-1:5000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human p27 KIP 1 protein |
| Abbre | p27 KIP 1 |
| Synonyms | KIP, MEN, CDKN, Cyclin-Dependent Kinase Inhibitor p, P27KIP, p27 KIP, CDKN1B, CDKN4, KIP1, MEN1B, MEN4, P27KIP1, p27 KIP 1, Cyclin-Dependent Kinase Inhibitor 1B, Cyclin-Dependent Kinase Inhibitor p27, AA408329, AI843786, Cdki1b, CDKN 1B, CDKN 4, CDN1B, Cyclin Dependent Kinase Inhibitor 1B, Cyclin dependent kinase inhibitor p27, Cyclin-dependent kinase inhibitor 1B (p27, Cyclin-dependent kinase inhibitor p27 Kip1, KIP 1, Kip1), OTTHUMP00000195098, OTTHUMP00000195099, p27, p27 Kip1, P27-like cyclin-dependent kinase inhibitor, p27 |
| Swissprot | |
| Calculated MW | 27 kDa |
| Observed MW |
27 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cell Biology, Epigenetics and Nuclear Signaling, Cancer |
| Clone No. | 3P8 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). |
Other Clones
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Unconjugated
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