Recombinant PAP Monoclonal Antibody (AN301619L)
-
-
-
- +1
For research use only.
| Verified Samples |
Verified Samples in WB: Human prostate Verified Samples in IHC: Human prostate hyperplasia, Human liver(Negative tissue) Verified Samples in FCM: HeLa |
| Dilution | WB 1:500-1:1000, IHC 1:50-1:100, FCM 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC, FCM |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human PAP fragment |
| Abbre | PAP |
| Synonyms | ACP, ACPP, 5'-NT, ACP-3, ACP3, 5'-Nucleotidase, Acid phosphatase prostate, ACP 3, Ecto-5'-Nucleotidase, PAP, PPAP, Prostatic Acid Phosphatase, Prostatic acid phosphotase, PSAP, Thiamine Monophosphatase, TMPase, 5'-NT, 5'-nucleotidase, Acid phosphatase prostate, ACP 3, ACP3, acpP, Ecto-5'-nucleotidase, PAP, PPAP, Prostatic acid phosphatase, Prostatic acid phosphotase, Thiamine monophosphatase, TMPase, acid phosphatase, prostate |
| Swissprot | |
| Calculated MW | 45 kDa |
| Observed MW |
50 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted, Lysosome memebrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Tags & Cell Markers, Cell Biology, Cancer |
| Clone No. | A322 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. |
Other Clones
{{antibodyDetailsPage.numTotal}} Results
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
Other Formats
{{formatDetailsPage.numTotal}} Results
Unconjugated
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.organization}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
