Recombinant PCSK9 Monoclonal Antibody (AN301958L)
For research use only.
| Verified Samples | Verified Samples in WB: F9, SH-SY5Y, BxPC-3, BRL |
| Dilution | WB 1:1000-1:5000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human PCSK9 fragment |
| Abbre | PCSK9 |
| Synonyms | PCSK, PSEC, Proprotein Convertase, Subtilisin/Kexin-Like Protease PC, Neural Apoptosis-Regulated Convertase, Proprotein Convertase Subtilisin/Kexin Type, LDLCQ, HCHOLA, PCSK9, FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, Neural Apoptosis-Regulated Convertase 1, Proprotein Convertase 9, Proprotein Convertase Subtilisin/Kexin Type 9, Subtilisin/Kexin-Like Protease PC9, PSEC0052, Neural apoptosis-regulated convert |
| Swissprot | |
| Calculated MW | 74 kDa |
| Observed MW |
90 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Cardiovascular, Stem Cells, Cell Biology, Metabolism |
| Clone No. | A674 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | PCSK9, also named as NARC1 and PC9, belongs to the peptidase S8 family. PCSK9 is expressed and secreted by several tissues but principally by the liver, small intestines, and kidney. It may be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis. PCSK9 is a regulator of the LDL receptor and plasma cholesterol concentrations. |
Other Clones
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Unconjugated
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