Recombinant PD-L1 Monoclonal Antibody (AN301623L)
For research use only.
| Verified Samples |
Verified Samples in WB: U87MG Verified Samples in IHC: Human cervical cancer, Human tonsil |
| Dilution | WB 1:500-1:2000, IHC 1:200-1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human PD-L1 fragment |
| Abbre | PD-L1 |
| Synonyms | PDL, B7H, PD-L, PD1L, Programmed cell death 1 ligand, PDCD1L, PDCD1LG, B7-H, B7H1, PDL1, PD-L1, PDCD1L1, PDCD1LG1, CD274, B7-H1, B7 homolog 1, CD274 molecule, PDCD1 ligand 1, Programmed death ligand 1, PD1L1, Programmed cell death 1 ligand 1, PDL1, PD-L1, B7-H, B7H1, CD274 molecule, PDCD1L1, PDCD1LG1, B7 H, B7 H1, CD 274, CD274 antigen, MGC142294, MGC142296, OTTHUMP00000021029, PD L1, PDL 1, RGD1566211 |
| Swissprot | |
| Calculated MW | 33 kDa |
| Observed MW |
50 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Plasma membrane, Endosome |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Immunology, Cancer |
| Clone No. | A326 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | PD-1 is an immunoinhibitory receptor expressed by activated T cells, B cells, and myeloid cells. Engagement of PD-1 by PD-L1 leads to the inhibition of T cell receptor–mediated lymphocyte proliferation and cytokine secretion. In addition, PD-1 signaling can inhibit at least suboptimal levels of CD28-mediated costimulation. PD-L1 is expressed by antigen-presenting cells, including human peripheral blood monocytes stimulated with interferong, and activated human and murine dendritic cells. |
Other Clones
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Other Formats
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Unconjugated
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