Recombinant PDK4 Monoclonal Antibody (AN302015L)
For research use only.
| Verified Samples | Verified Samples in WB: Mouse heart, Rat heart |
| Dilution | WB 1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Rat, Mouse |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Peptide. This information is proprietary to PTMab. |
| Abbre | PDK4 |
| Synonyms | PDK, PDHK, Pyruvate dehydrogenase lipoamide kinase isozyme, PDK4 PDHK, PDK4, FLJ40832, mitochondrial, Pyruvate dehydrogenase lipoamide kinase isozyme 4, PDK4 PDHK4, PDHK4, [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, Pyruvate dehydrogenase [lipoamide] kinase isozyme 4 mitochondrial, Pyruvate dehydrogenase kinase 4, Pyruvate dehydrogenase kinase isoenzyme 4, Pyruvate dehydrogenase kinase isoform 4, Pyruvate dehydrogenase kinase isozyme 4, Pyruvate dehydrogenase kinase isozyme 4 mitochondrial |
| Swissprot | |
| Calculated MW | 46 kDa |
| Observed MW |
44 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Mitochondrion matrix |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Metabolism |
| Clone No. | A735 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Pyruvate dehydrogenase kinase isoform4 (PDK4) is also named as PDHK4 and belongs to the PDK/BCKDK protein kinase family. It is upregulated by starvation in many tissues of the body during starvation. This causes inactivation of the pyruvate dehydrogenase complex which blocks pyruvate oxidation and conserves lactate and alanine for gluconeogenesis. Enhanced PDK4 expression may be caused by the increase in free fatty acids that occurs during starvation. Free fatty acids can activate peroxisome proliferator-activated receptor α (PPARα), and activation of PPARα can promote PDK4 expression. |
Other Clones
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Unconjugated
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