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Recombinant PELP1 Monoclonal Antibody - 1
  • Recombinant PELP1 Monoclonal Antibody - 1
  • Recombinant PELP1 Monoclonal Antibody - 2
  • Recombinant PELP1 Monoclonal Antibody - 3
All Size Price Qty
100μL $ 380.00
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50μL $ 249.00
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For research use only.

Verified Samples Verified Samples in WB: HeLa, MCF-7, HEK-293, T47D
Verified Samples in IHC: Human breast cancer
Dilution WB 1:500-1:1000,  IHC 1:50-1:100
Isotype IgG, κ
Host Rabbit
Reactivity Human,  
Applications WB,  IHC
Clonality Monoclonal;Recombinant
Immunogen Recombinant human PELP1 fragment
Abbre PELP1
Synonyms HMX,  PELP,  PELP1,  MNAR,  P160,  proline,  HMX3
Swissprot
Calculated MW 120 kDa
Observed MW 160 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cytoplasm, Nucleus
Concentration 1 mg/mL
Buffer PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Purification Method Protein A purified
Research Areas Signal Transduction,  Epigenetics and Nuclear Signaling
Clone No. A328
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background Coactivator of estrogen receptor-mediated transcription and a corepressor of other nuclear hormone receptors and sequence-specific transcription factors. Plays a role in estrogen receptor (ER) genomic activity when present in the nuclear compartment by activating the ER target genes in a hormonal stimulation dependent manner. Can facilitate ER non-genomic signaling via SRC and PI3K interaction in the cytosol. Plays a role in E2-mediated cell cycle progression by interacting with RB1.
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Unconjugated

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