Recombinant Phospho-BRD2 (S37) Monoclonal Antibody (AN302085L)
For research use only.
| Verified Samples | Verified Samples in WB: HEK-293T, HEK-293T + Calyculin A (100 nM, 60 min) |
| Dilution | WB 1:4000-1:20000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human BRD2 (phospho S37) fragment |
| Abbre | Phospho-BRD2 (S37) |
| Synonyms | RNF, BRD, FSRG, RING, KIAA, BRD2-IT, BRD2, BRD2-IT1, D6S113E, FSH, FSRG1, NAT, O27.1.1, RING3, RNF3, KIAA9001 |
| Swissprot | |
| Calculated MW | 88 kDa |
| Observed MW |
110 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cell Biology, Signal Transduction, Epigenetics and Nuclear Signaling |
| Clone No. | A809 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Brd2 is a highly conserved member of the BET subfamily of bromodomain proteins that contain two tandem N-terminal bromodomains and a single C-terminal extra-terminal (ET) domain. In addition to its involvement in guiding the expression of cell cycle genes through its binding to multiple E2Fs, Brd2 has been shown to be associated with several regulators of transcription, including TFIID and Swi/Snf complexes. First identified as a nuclear serine/threonine kinase, Brd2, like other bromodomain proteins, is thought to function in mammalian development by regulating chromatin structure and transcription. Brd2 has been shown to bind to histone H4 via acetylated Lys12, a substrate of several histone acetyltransferase transcriptional coactivators. |
Other Clones
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Unconjugated
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