Recombinant Phospho-CD31 (Tyr713) Monoclonal Antibody (AN302093L)
For research use only.
| Verified Samples | Verified Samples in WB: Jurkat |
| Dilution | WB 1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | phosphorylated human CD31 (Tyr713) peptide |
| Abbre | Phospho-CD31 (Tyr713) |
| Synonyms | PECA, cluster of differentiation, PECAM1, CD31, CD31/EndoCAM, GPIIA, PECA1, PECAM-1, endoCAM, cluster of differentiation 31, Platelet endothelial cell adhesion molecule, endocam, Adhesion molecule, CD31 antigen, CD31 EndoCAM, FLJ34100, FLJ58394, GPIIA', PECAM, Pecam 1, PECAM 1 CD31 EndoCAM, PECAM1, Platelet and endothelial cell adhesion molecule 1, Platelet/endothelial cell adhesion molecule 1, CD31, EndoCAM, GPIIA, PECA1, PECAM-1, Platelet endothelial cell adhesion molecule |
| Swissprot | |
| Calculated MW | 83 kDa |
| Observed MW |
130 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cardiovascular, Signal Transduction, Stem Cells, Cancer |
| Clone No. | A817 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | CD31, also named as Platelet Endothelial Cell Adhesion Molecule-1 (PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells, and endothelial cells and modulates cell adhesion, endothelial cell migration, and angiogenesis. CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical or oxidative stress), presumably by Src family members. The tyrosine phosphorylation mediates associations with a number of SH2 domain-containing binding partners such as PI3 kinase, SHIP, PLCγ, and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules. |
Other Clones
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Unconjugated
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