Recombinant Phospho-GSK3 α, β (Tyr216, Tyr279) Monoclonal Antibody (AN300656L)
For research use only.
| Verified Samples | Verified Samples in WB: C6 |
| Dilution | WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | A synthetic peptide corresponding to residues around (Tyr216, Tyr279)of Human Phospho-GSK3 α, β |
| Abbre | GSK3 α, β |
| Synonyms | DKFZp686D, GSK3 alpha, GSK3A, DKFZp686D0638, Glycogen synthase kinase 3 alpha, Glycogen synthase kinase-3 alpha, GSK 3 alpha, GSK 3A, GSK-3 alpha, GSK3alpha, Serine/threonine protein kinase GSK3A, Serine/threonine-protein kinase GSK3A, glycogen synthase kinase-3 alpha, 2700086H06Rik, GSK3α/β |
| Swissprot | |
| Calculated MW | 47 kDa,51 kDa |
| Observed MW |
47 kDa,51 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Neuroscience, Signal Transduction, Stem Cells, Cell Biology, Cardiovascular |
| Clone No. | 1F8 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | GSK3A(glycogen synthase kinase 3 alpha) Homo sapiens This gene encodes a multifunctional Ser/Thr protein kinase that is implicated inThe control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role inThe WNT and PI3K signaling pathways, as well as regulatesThe production of beta-amyloid peptides associated with Alzheimer's disease. |
Other Clones
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Unconjugated
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